Dynamica Halo RB-10 Spectrophotometer
The Halo RB-10 is a ratio beam spectrophotometer with an extensive array of built in functions for versatility and suitability to many analytical and biological applications. The ratio beam optics impart greater accuracy and reproducibility than the conventional, single beam optics.
Ratio Beam Optics
The absorbance signal in conventional single beam instruments can destabilize even during simple applications thus resulting in data inaccuracy. By contrast, ratio beam optics compensate and stabilize any signal fluctuations to increase accuracy and reliability even over prolonged usage such as in time course and kinetic measurements. The ratio beam principle involves splitting the beam generated by the light source by a half mirror. One of the split beams passes through the sample and is quantified by a detector whereas the other split beam which is representative of the absorbance signal is measured by an independent detector to obtain a signal reference. The ratio of the values from both detectors is then calculated to detect and compensate for any aberration in the energy of the light source or a temporal change of the optical elements and produce the highly stable photometric values.
Boasting a 2 nm spectral bandpass the Halo RB-10 offers superior spectra and peak resolution. Other specifications include an impressive wavelength accuracy of ±0.5nm, noise level 0.0005Abs (500nm) and stray light ≤0.05% (220nm NaI, 340nm NaNO2).
Built-in and Diverse Range of Measurement Modes
Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single wavelength, up to 6 multiple individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves with up to 20 standards for concentration measurements.
Time Scan: Perform kinetic measurements for time periods ranging from 1 minute to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.
Wavelength scan: Perform a full spectral scan from 190 to 1,100nm at any of 8 incremental and preset selectable scan speeds starting from a high resolution 10nm/minute up to a swift 3,600nm /minute. Data is displayed as either numerical values or a graphical spectrum. Furthermore perform downstream processing of data, such as peak / valley search or smoothing, directly on board or with the optional Halo UV Detective software.
Dual Lamp Advantage
By virtue of the halogen tungsten and deuterium lamps typically found in higher end, analytical spectrophotometers the Halo RB-10 wavelength range is an impressive 190nm – 1,100nm. Furthermore a dual lamp system results in higher accuracy than corresponding xenon lamps. Lamp switching is automatic (by default at 340nm) and both lamps are long life.
User friendly operation and information rich LCD display
The 94mm x 70mm, backlit LCD screen with adjustable brightness control is sufficiently large to display a large array of data even in a graphical format. Furthermore single wavelength absorbance or transmittance data can be enhanced and enlarged using the unique zoom function. The seamless and chemical resistant keypad is designed for easy and quick selection of navigation and function features whilst protecting against any laboratory spills. Other unique features include the ‘GO TO WL’ short cut key to allow direct input of a new wavelength into an existing measurement.
To ensure optimum instrument performance, a self-diagnostic function incorporating a number of parameters is executed each time the Halo RB-10 is switched on. Furthermore the Halo RB-10 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can be all validated and the audit report printed.
Stand alone or PC Operation
The Halo RB-10 is fully equipped and capable of executing all functions in stand alone mode. Simply connect a standard laser printer for direct printouts of data and graphs. For more advanced analyses and reporting, the simple slide of a switch places the Halo RB-10 under the direct control of the optional UV Detective software installed on a computer with Windows® XP or Windows® 7 operating system.
4 Sample Cuvette Holder
A cuvette holder / changer with a 4 cuvette capacity is supplied as standard. Therefore measurement can be expedited by inserting the 4 cuvettes in tandem and manually sliding the holder / changer forwards or backwards to select the appropriate cuvette for measurement. The cuvette holder /changer is easily removed for cleaning purposes.
Specifications Dynamica Halo RB-10 Spectrophotometer
|Optics||Concave diffraction grating / Ratio beam Principle|
|Wavelength Range||190nm -1,100 nm|
|Stray Light||≤0.05% (220nm NaI, 340nm NaNO2|
|Photometric Range||Absorbance: -3 to +3 %T: 0% to 300% Concentration: 0,000 to 9,999|
|Wavelength Scan Speed||10, 100, 200, 400, 800, 1,200, 2,400, 3,600 nm/minute|
|Baseline Stability||0.001 Abs/hr (500nm, after 2 hours)|
|Noise Level||0.0005 Abs (500nm)|
|Light Source||Tungsten-Halogen and Deuterium Lamps|
|Light Source Switching||Automatic switching at 340nm|
|Display||Back-lit LCD 94(W) x 70(H) mm|
|Dimensions||370(W) x 550(D) x 265(H) mm|
|Power Requirements||110 – 220 V selectable, 50/60Hz|
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